Review





Similar Products

premo autophagy tandem sensor rfp-gfp-lc3b kit p36239  (Thermo Fisher)
90
Thermo Fisher premo autophagy tandem sensor rfp-gfp-lc3b kit p36239
Premo Autophagy Tandem Sensor Rfp Gfp Lc3b Kit P36239, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/premo autophagy tandem sensor rfp-gfp-lc3b kit p36239/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
premo autophagy tandem sensor rfp-gfp-lc3b kit p36239 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
premo autophagy tandem sensor rfp-gfp-lc3b kit # p36239  (Thermo Fisher)
90
Thermo Fisher premo autophagy tandem sensor rfp-gfp-lc3b kit # p36239
Premo Autophagy Tandem Sensor Rfp Gfp Lc3b Kit # P36239, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/premo autophagy tandem sensor rfp-gfp-lc3b kit # p36239/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
premo autophagy tandem sensor rfp-gfp-lc3b kit # p36239 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
fastscan 1× cell extraction buffer  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc fastscan 1× cell extraction buffer
Fastscan 1× Cell Extraction Buffer, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fastscan 1× cell extraction buffer/product/Cell Signaling Technology Inc
Average 94 stars, based on 1 article reviews
fastscan 1× cell extraction buffer - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
fastscan total lc3b elisa kit  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc fastscan total lc3b elisa kit
Figure 2. Validation of the autophagy measurement methodology A, MAP1LC3B was knocked out using CRISPR-Cas9 gene editing. A western blot for <t>LC3B</t> shows complete loss of LC3B-I and LC3B-II bands around 15 kDa. B, quantification of three independent cell lysates from wild-type and LC3B KO cells without and with CQ on the same <t>ELISA</t> that was used for the main analysis of this study shows very high specificity for the target protein. An unpaired t test was used to test significance. C, three individual vacutainers containing blood were taken from a participant and processed in parallel. Resulting cell pellets were saponin washed in parallel and both the saponin-washed pellet (P: containing lipid-bound proteins such as lipidated LC3B-II) and the saponin wash supernatant (S: containing soluble cytosolic proteins such as non-lipidated LC3B-I) are shown. Western blots for LC3B show LC3B-II that generally runs just below 15 kDa in the pellet, and LC3B-I that generally runs just above 15 kDa in the supernatant. Total protein staining is also shown to illustrate the consistency of the saponin extraction and the contrasting protein content of the saponin-soluble (S) and saponin-insoluble (P) fractions. D, three different blood tubes were taken from an individual and processed in parallel. Resulting cell pellets were processed by ELISA. LC3B in saponin-washed cell pellets (three tubes from five individuals, with or without CQ) were quantified by ELISA and grouped appropriately. Addition of CQ causes increases in LC3B-II. Significance was quantified using multiple t tests and P-values are adjusted for multiple measures. Bars = mean ± SD for three parallel processed samples for each participant. Parallel samples appear as datapoints around the bars. E, variation for each participant presented in D, with or without CQ, is displayed as the coefficient of variation (CV).
Fastscan Total Lc3b Elisa Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fastscan total lc3b elisa kit/product/Cell Signaling Technology Inc
Average 94 stars, based on 1 article reviews
fastscan total lc3b elisa kit - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
autophagy tandem rfp-gfp-lc3b kit  (Thermo Fisher)
90
Thermo Fisher autophagy tandem rfp-gfp-lc3b kit
Figure 2. Validation of the autophagy measurement methodology A, MAP1LC3B was knocked out using CRISPR-Cas9 gene editing. A western blot for <t>LC3B</t> shows complete loss of LC3B-I and LC3B-II bands around 15 kDa. B, quantification of three independent cell lysates from wild-type and LC3B KO cells without and with CQ on the same <t>ELISA</t> that was used for the main analysis of this study shows very high specificity for the target protein. An unpaired t test was used to test significance. C, three individual vacutainers containing blood were taken from a participant and processed in parallel. Resulting cell pellets were saponin washed in parallel and both the saponin-washed pellet (P: containing lipid-bound proteins such as lipidated LC3B-II) and the saponin wash supernatant (S: containing soluble cytosolic proteins such as non-lipidated LC3B-I) are shown. Western blots for LC3B show LC3B-II that generally runs just below 15 kDa in the pellet, and LC3B-I that generally runs just above 15 kDa in the supernatant. Total protein staining is also shown to illustrate the consistency of the saponin extraction and the contrasting protein content of the saponin-soluble (S) and saponin-insoluble (P) fractions. D, three different blood tubes were taken from an individual and processed in parallel. Resulting cell pellets were processed by ELISA. LC3B in saponin-washed cell pellets (three tubes from five individuals, with or without CQ) were quantified by ELISA and grouped appropriately. Addition of CQ causes increases in LC3B-II. Significance was quantified using multiple t tests and P-values are adjusted for multiple measures. Bars = mean ± SD for three parallel processed samples for each participant. Parallel samples appear as datapoints around the bars. E, variation for each participant presented in D, with or without CQ, is displayed as the coefficient of variation (CV).
Autophagy Tandem Rfp Gfp Lc3b Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/autophagy tandem rfp-gfp-lc3b kit/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
autophagy tandem rfp-gfp-lc3b kit - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
premotm autophagy tandem sensor rfp-gfp-lc3b kit p36239  (Thermo Fisher)
90
Thermo Fisher premotm autophagy tandem sensor rfp-gfp-lc3b kit p36239
Figure 2. Validation of the autophagy measurement methodology A, MAP1LC3B was knocked out using CRISPR-Cas9 gene editing. A western blot for <t>LC3B</t> shows complete loss of LC3B-I and LC3B-II bands around 15 kDa. B, quantification of three independent cell lysates from wild-type and LC3B KO cells without and with CQ on the same <t>ELISA</t> that was used for the main analysis of this study shows very high specificity for the target protein. An unpaired t test was used to test significance. C, three individual vacutainers containing blood were taken from a participant and processed in parallel. Resulting cell pellets were saponin washed in parallel and both the saponin-washed pellet (P: containing lipid-bound proteins such as lipidated LC3B-II) and the saponin wash supernatant (S: containing soluble cytosolic proteins such as non-lipidated LC3B-I) are shown. Western blots for LC3B show LC3B-II that generally runs just below 15 kDa in the pellet, and LC3B-I that generally runs just above 15 kDa in the supernatant. Total protein staining is also shown to illustrate the consistency of the saponin extraction and the contrasting protein content of the saponin-soluble (S) and saponin-insoluble (P) fractions. D, three different blood tubes were taken from an individual and processed in parallel. Resulting cell pellets were processed by ELISA. LC3B in saponin-washed cell pellets (three tubes from five individuals, with or without CQ) were quantified by ELISA and grouped appropriately. Addition of CQ causes increases in LC3B-II. Significance was quantified using multiple t tests and P-values are adjusted for multiple measures. Bars = mean ± SD for three parallel processed samples for each participant. Parallel samples appear as datapoints around the bars. E, variation for each participant presented in D, with or without CQ, is displayed as the coefficient of variation (CV).
Premotm Autophagy Tandem Sensor Rfp Gfp Lc3b Kit P36239, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/premotm autophagy tandem sensor rfp-gfp-lc3b kit p36239/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
premotm autophagy tandem sensor rfp-gfp-lc3b kit p36239 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
premotm autophagy tandem sensor rfp-gfp-lc3b kit  (Thermo Fisher)
90
Thermo Fisher premotm autophagy tandem sensor rfp-gfp-lc3b kit
Figure 2. Validation of the autophagy measurement methodology A, MAP1LC3B was knocked out using CRISPR-Cas9 gene editing. A western blot for <t>LC3B</t> shows complete loss of LC3B-I and LC3B-II bands around 15 kDa. B, quantification of three independent cell lysates from wild-type and LC3B KO cells without and with CQ on the same <t>ELISA</t> that was used for the main analysis of this study shows very high specificity for the target protein. An unpaired t test was used to test significance. C, three individual vacutainers containing blood were taken from a participant and processed in parallel. Resulting cell pellets were saponin washed in parallel and both the saponin-washed pellet (P: containing lipid-bound proteins such as lipidated LC3B-II) and the saponin wash supernatant (S: containing soluble cytosolic proteins such as non-lipidated LC3B-I) are shown. Western blots for LC3B show LC3B-II that generally runs just below 15 kDa in the pellet, and LC3B-I that generally runs just above 15 kDa in the supernatant. Total protein staining is also shown to illustrate the consistency of the saponin extraction and the contrasting protein content of the saponin-soluble (S) and saponin-insoluble (P) fractions. D, three different blood tubes were taken from an individual and processed in parallel. Resulting cell pellets were processed by ELISA. LC3B in saponin-washed cell pellets (three tubes from five individuals, with or without CQ) were quantified by ELISA and grouped appropriately. Addition of CQ causes increases in LC3B-II. Significance was quantified using multiple t tests and P-values are adjusted for multiple measures. Bars = mean ± SD for three parallel processed samples for each participant. Parallel samples appear as datapoints around the bars. E, variation for each participant presented in D, with or without CQ, is displayed as the coefficient of variation (CV).
Premotm Autophagy Tandem Sensor Rfp Gfp Lc3b Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/premotm autophagy tandem sensor rfp-gfp-lc3b kit/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
premotm autophagy tandem sensor rfp-gfp-lc3b kit - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
premo autophagy tandem sensor rfp-gfp-lc3b kit  (Thermo Fisher)
90
Thermo Fisher premo autophagy tandem sensor rfp-gfp-lc3b kit
Figure 2. Validation of the autophagy measurement methodology A, MAP1LC3B was knocked out using CRISPR-Cas9 gene editing. A western blot for <t>LC3B</t> shows complete loss of LC3B-I and LC3B-II bands around 15 kDa. B, quantification of three independent cell lysates from wild-type and LC3B KO cells without and with CQ on the same <t>ELISA</t> that was used for the main analysis of this study shows very high specificity for the target protein. An unpaired t test was used to test significance. C, three individual vacutainers containing blood were taken from a participant and processed in parallel. Resulting cell pellets were saponin washed in parallel and both the saponin-washed pellet (P: containing lipid-bound proteins such as lipidated LC3B-II) and the saponin wash supernatant (S: containing soluble cytosolic proteins such as non-lipidated LC3B-I) are shown. Western blots for LC3B show LC3B-II that generally runs just below 15 kDa in the pellet, and LC3B-I that generally runs just above 15 kDa in the supernatant. Total protein staining is also shown to illustrate the consistency of the saponin extraction and the contrasting protein content of the saponin-soluble (S) and saponin-insoluble (P) fractions. D, three different blood tubes were taken from an individual and processed in parallel. Resulting cell pellets were processed by ELISA. LC3B in saponin-washed cell pellets (three tubes from five individuals, with or without CQ) were quantified by ELISA and grouped appropriately. Addition of CQ causes increases in LC3B-II. Significance was quantified using multiple t tests and P-values are adjusted for multiple measures. Bars = mean ± SD for three parallel processed samples for each participant. Parallel samples appear as datapoints around the bars. E, variation for each participant presented in D, with or without CQ, is displayed as the coefficient of variation (CV).
Premo Autophagy Tandem Sensor Rfp Gfp Lc3b Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/premo autophagy tandem sensor rfp-gfp-lc3b kit/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
premo autophagy tandem sensor rfp-gfp-lc3b kit - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


Figure 2. Validation of the autophagy measurement methodology A, MAP1LC3B was knocked out using CRISPR-Cas9 gene editing. A western blot for LC3B shows complete loss of LC3B-I and LC3B-II bands around 15 kDa. B, quantification of three independent cell lysates from wild-type and LC3B KO cells without and with CQ on the same ELISA that was used for the main analysis of this study shows very high specificity for the target protein. An unpaired t test was used to test significance. C, three individual vacutainers containing blood were taken from a participant and processed in parallel. Resulting cell pellets were saponin washed in parallel and both the saponin-washed pellet (P: containing lipid-bound proteins such as lipidated LC3B-II) and the saponin wash supernatant (S: containing soluble cytosolic proteins such as non-lipidated LC3B-I) are shown. Western blots for LC3B show LC3B-II that generally runs just below 15 kDa in the pellet, and LC3B-I that generally runs just above 15 kDa in the supernatant. Total protein staining is also shown to illustrate the consistency of the saponin extraction and the contrasting protein content of the saponin-soluble (S) and saponin-insoluble (P) fractions. D, three different blood tubes were taken from an individual and processed in parallel. Resulting cell pellets were processed by ELISA. LC3B in saponin-washed cell pellets (three tubes from five individuals, with or without CQ) were quantified by ELISA and grouped appropriately. Addition of CQ causes increases in LC3B-II. Significance was quantified using multiple t tests and P-values are adjusted for multiple measures. Bars = mean ± SD for three parallel processed samples for each participant. Parallel samples appear as datapoints around the bars. E, variation for each participant presented in D, with or without CQ, is displayed as the coefficient of variation (CV).

Journal: The Journal of Physiology

Article Title: Intermittent time‐restricted eating may increase autophagic flux in humans: an exploratory analysis

doi: 10.1113/jp287938

Figure Lengend Snippet: Figure 2. Validation of the autophagy measurement methodology A, MAP1LC3B was knocked out using CRISPR-Cas9 gene editing. A western blot for LC3B shows complete loss of LC3B-I and LC3B-II bands around 15 kDa. B, quantification of three independent cell lysates from wild-type and LC3B KO cells without and with CQ on the same ELISA that was used for the main analysis of this study shows very high specificity for the target protein. An unpaired t test was used to test significance. C, three individual vacutainers containing blood were taken from a participant and processed in parallel. Resulting cell pellets were saponin washed in parallel and both the saponin-washed pellet (P: containing lipid-bound proteins such as lipidated LC3B-II) and the saponin wash supernatant (S: containing soluble cytosolic proteins such as non-lipidated LC3B-I) are shown. Western blots for LC3B show LC3B-II that generally runs just below 15 kDa in the pellet, and LC3B-I that generally runs just above 15 kDa in the supernatant. Total protein staining is also shown to illustrate the consistency of the saponin extraction and the contrasting protein content of the saponin-soluble (S) and saponin-insoluble (P) fractions. D, three different blood tubes were taken from an individual and processed in parallel. Resulting cell pellets were processed by ELISA. LC3B in saponin-washed cell pellets (three tubes from five individuals, with or without CQ) were quantified by ELISA and grouped appropriately. Addition of CQ causes increases in LC3B-II. Significance was quantified using multiple t tests and P-values are adjusted for multiple measures. Bars = mean ± SD for three parallel processed samples for each participant. Parallel samples appear as datapoints around the bars. E, variation for each participant presented in D, with or without CQ, is displayed as the coefficient of variation (CV).

Article Snippet: To determine LC3B-II sample concentration, 5 μg per well (diluted in CST FastScan 1× Cell Extraction Buffer) of sample was loaded in triplicate on a FastScan Total LC3B ELISA Kit (Cell Signaling Technology, 35172).

Techniques: Biomarker Discovery, CRISPR, Western Blot, Enzyme-linked Immunosorbent Assay, Staining, Extraction